Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123218_e43d TGGGGCAGAGTTGCCCACTCCGACAGTCCTCG(+AAAAAA)GATCCCCGCGCCCTGGGCGCGGTTCACACCG NGG 10 8 17 8 24 0 100 5 10
    240606123216_r6d1 CCGGCCGGTGTGAACCGCGCCCAGGGCGCGGG(+AAAAAA)GATCCGAGGACTGTCGGAGTGGGCAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240606123215_09g0 TTATCTCTGCGTAGTCCAAAACAAAGTAAATG(+AAAAAA)GTACCGCTGTCGTGCTGCGAAGGTGAGAACG NGG 10 8 17 8 24 0 100 5 10
    240606123213_auwa ACTAGCGTTCTCACCTTCGCAGCACGACAGCG(+AAAAAA)GTACCATTTACTTTGTTTTGGACTACGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606123212_nulk CTCCAAGCCAGCTGGGCCATCAGATGCTAATG(+AAAAAA)GTACCCAGAAATCCCTCACTTGTCATCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606123211_x72z AATCCACAGGATGACAAGTGAGGGATTTCTGG(+AAAAAA)GTACCATTAGCATCTGATGGCCCAGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606123209_3u23 AGGGTAAGGAAATCTACTTGCCCTACCTGTGG(+AAAAAA)GATCCAGGCCTGGTTAAAATGGTACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240606123207_i3zd TGCTAAGTCAAGTACCATTTTAACCAGGCCTG(+AAAAAA)GATCCCACAGGTAGGGCAAGTAGATTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606123204_3c9q GATCAGCTTGCCCTGCTATGTACCATGTGGGG(+AAAAAA)GATCCAGGGCTGAACTCAGGTTGTCAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606123201_z3lp CTGCCAAGCCTGACAACCTGAGTTCAGCCCTG(+AAAAAA)GATCCCCCACATGGTACATAGCAGGGCAAGC NGG 10 8 17 8 24 0 100 5 10