Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123234_bfh6 ATGGGCTAGTGTCTTTTGAGAGGAAATCAGTG(+AAAAAA)GATCCCGGATTTGCTCCTGTTAATGAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606123231_i7qy CTTTAAAACTTCATTAACAGGAGCAAATCCGG(+AAAAAA)GATCCACTGATTTCCTCTCAAAAGACACTAG NGG 10 8 17 8 24 0 100 5 10
    240606123230_r90k AGGAACATTTTAAAGTTCCAACTATGGGAAAG(+AAAAAA)GTACCTGATACGGGGTTTCCCTGCAAATTTA NGG 10 8 17 8 24 0 100 5 10
    240606123229_yyiw ATGTCTAAATTTGCAGGGAAACCCCGTATCAG(+AAAAAA)GTACCTTTCCCATAGTTGGAACTTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606123228_xoew CCTTGTGCTTAGCTTCCTGGGAGGAGTGAACG(+AAAAAA)GTACCTGCCCTTCTCAGTGCTCCTGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240606123226_petb CGTGATACTCACAGGAGCACTGAGAAGGGCAG(+AAAAAA)GTACCGTTCACTCCTCCCAGGAAGCTAAGCA NGG 10 8 17 8 24 0 100 5 10
    240606123225_03dq TAAAACACCATTTTGTTTGGTGCTAGAGATAG(+AAAAAA)GATCCAGGCTTTGTCCATGCCAGGCAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606123223_64qd GAGGAGTGCCTGCCTGGCATGGACAAAGCCTG(+AAAAAA)GATCCTATCTCTAGCACCAAACAAAATGGTG NGG 10 8 17 8 24 0 100 5 10
    240606123221_zku9 TGTTTTAAGATTTTGAAAGGGAATTGTTGGTG(+AAAAAA)GATCCATTTGATATTTAATTTTATTTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240606123220_uuaq TTTAACCAAAAAATAAAATTAAATATCAAATG(+AAAAAA)GATCCACCAACAATTCCCTTTCAAAATCTTA NGG 10 8 17 8 24 0 100 5 10