Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123129_mwoj GACCTGAATTCTCCACACAGTTGATCCTGCTG(+AAAAAA)GATCCCACACATGAGAATCATATAAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240606123127_pmbn CAGGACAGTTCTTATATGATTCTCATGTGTGG(+AAAAAA)GATCCAGCAGGATCAACTGTGTGGAGAATTC NGG 10 8 17 8 24 0 100 5 10
    240606123125_8ox3 TGTCCTTCCCACAGCCTCCTGGGCTGGTGCCG(+AAAAAA)GATCCGGGAAGGATGAGTTATCATCTAACCT NGG 10 8 17 8 24 0 100 5 10
    240606123123_b4xc GCTGTAGGTTAGATGATAACTCATCCTTCCCG(+AAAAAA)GATCCGGCACCAGCCCAGGAGGCTGTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606123121_bqpq AGATCCTCCTGCCTCTGCCTCCCAAGATCTGG(+AAAAAA)GATCCACACCCAGCTGTCCGTTATGTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606123120_s14d AAAGGATTTTACATAACGGACAGCTGGGTGTG(+AAAAAA)GATCCCAGATCTTGGGAGGCAGAGGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606123118_a30s AGTGTATCTTCAAGAGACAAGAACACTTGCTG(+AAAAAA)GATCCGTTCACAAGGGTGAGGTTTAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240606123117_sdtd CTCTCAGTTGCTAAACCTCACCCTTGTGAACG(+AAAAAA)GATCCAGCAAGTGTTCTTGTCTCTTGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606123115_d0v6 GAAGCATTAGATCCTGTCCTGAAGGAGTCTGG(+AAAAAA)GTACCCACTTAGTGTCCTCTGGTCAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240606123114_eaee TAGAAACATTTTGACCAGAGGACACTAAGTGG(+AAAAAA)GTACCCAGACTCCTTCAGGACAGGATCTAAT NGG 10 8 17 8 24 0 100 5 10