Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123041_dkge CCATTCATAGTTCCATAACTCCGGTTCTGTAG(+AAAAAA)GATCCGAGGTTCTTTTCTCACCCGTACGAAA NGG 10 8 17 8 24 0 100 5 10
    240606123039_wjnd ATGCATTTCGTACGGGTGAGAAAAGAACCTCG(+AAAAAA)GATCCTACAGAACCGGAGTTATGGAACTATG NGG 10 8 17 8 24 0 100 5 10
    240606123036_e9na TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606123035_ty4z TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606123033_gpf7 GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606123032_x0sp GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606123030_mou0 CTGTGCTGCCAGCCCCTGCCCAGGTTCCTTTG(+AAAAAA)GATCCGCAGATGAAAGACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240606123028_gkkx GTGTGTGTGTGTGTGTGTGTCTTTCATCTGCG(+AAAAAA)GATCCAAAGGAACCTGGGCAGGGGCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606123027_epm5 TTAATTTCACAGATTCTTGTTCCAATGTCTAG(+AAAAAA)GATCCATACATGATCTTTAGGGCTCCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606123026_esd0 GCCCTTTGAAGGAGCCCTAAAGATCATGTATG(+AAAAAA)GATCCTAGACATTGGAACAAGAATCTGTGAA NGG 10 8 17 8 24 0 100 5 10