Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123008_dqba GTCAGCCGCGTGTTCGAGCGGTGTCACAGCCG(+AAAAAA)GATCCTGCCCAGACTGCTGCCTGTGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606123006_m49o AAGGGAAAGCACACAGGCAGCAGTCTGGGCAG(+AAAAAA)GATCCGGCTGTGACACCGCTCGAACACGCGG NGG 10 8 17 8 24 0 100 5 10
    240606123004_jent GGACTGATCCGAAGACTCTATGCTGGAACCTG(+AAAAAA)GATCCTCATCATCTATGTATATAAGATCTTT NGG 10 8 17 8 24 0 100 5 10
    240606123003_kxbc TGCCAAAAGATCTTATATACATAGATGATGAG(+AAAAAA)GATCCAGGTTCCAGCATAGAGTCTTCGGATC NGG 10 8 17 8 24 0 100 5 10
    240606123002_lt7o CTGTAAGACGTTTGCTTTGTAACTTGGTCACG(+AAAAAA)GATCCAAGCCCATGCTGGGGGAAATGGCACT NGG 10 8 17 8 24 0 100 5 10
    240606123001_9036 GCACTAGTGCCATTTCCCCCAGCATGGGCTTG(+AAAAAA)GATCCGTGACCAAGTTACAAAGCAAACGTCT NGG 10 8 17 8 24 0 100 5 10
    240606122959_xv6r CTCAGTCTGCTCTCCACTGTCCATGTTGTTGG(+AAAAAA)GATCCGGCACAACCGAGCCTGTGTACTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606122957_b4sf GAGGGTCCCAGTACACAGGCTCGGTTGTGCCG(+AAAAAA)GATCCCAACAACATGGACAGTGGAGAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606122956_fds6 GTAAATATGCAAAAAGCGTTTCCTAATGGCTG(+AAAAAA)GATCCATGGTGTCTGGCCCAATGAAGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240606122954_t32f CTTTCTCTTACTTCATTGGGCCAGACACCATG(+AAAAAA)GATCCAGCCATTAGGAAACGCTTTTTGCATA NGG 10 8 17 8 24 0 100 5 10