Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122922_thv5 TCACTCAGAGTGGGGGACCTGTCCATACTGCG(+AAAAAA)GATCCTGCAGGCTCTGGGTGGGGACAGCACC NGG 10 8 17 8 24 0 100 5 10
    240606122921_g9gc GCCAGGGTGCTGTCCCCACCCAGAGCCTGCAG(+AAAAAA)GATCCGCAGTATGGACAGGTCCCCCACTCTG NGG 10 8 17 8 24 0 100 5 10
    240606122919_tauw TGAGTTTGCCAGGAGCCCTGGGGTGCAGGCAG(+AAAAAA)GATCCCTGCAGCCCCACAGCCAACTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606122917_2sbk GAAGAAGGAAAAGTTGGCTGTGGGGCTGCAGG(+AAAAAA)GATCCTGCCTGCACCCCAGGGCTCCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240606122916_tu9z AGCCGTGGAACACGTCTCTTAGGGTGTGCTTG(+AAAAAA)GATCCAGAGCCCTGCAGGTCTCTGGGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240606122915_uz32 AGTGACAAACCCCAGAGACCTGCAGGGCTCTG(+AAAAAA)GATCCAAGCACACCCTAAGAGACGTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606122913_e81w TCTCTCTATCCCTTAAAAAGAACTATCTCCAG(+AAAAAA)GTACCCAGTCCTGTTGTAGAAACCACCTGAT NGG 10 8 17 8 24 0 100 5 10
    240606122911_un1g TGGGCATCAGGTGGTTTCTACAACAGGACTGG(+AAAAAA)GTACCTGGAGATAGTTCTTTTTAAGGGATAG NGG 10 8 17 8 24 0 100 5 10
    240606122910_j7rq TTGTTCTCCAGACCTGACCCAGGTAGGCAAGG(+AAAAAA)GTACCCTCCCAGTCTCACCTACCATACTGTG NGG 10 8 17 8 24 0 100 5 10
    240606122908_g53s GATGGCACAGTATGGTAGGTGAGACTGGGAGG(+AAAAAA)GTACCCTTGCCTACCTGGGTCAGGTCTGGAG NGG 10 8 17 8 24 0 100 5 10