Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122938_03c3 CCTAGGTTATGAATGACAGCCACAGATGCTGG(+AAAAAA)GATCCCTATTGAGAAGGCAGTGCTATCGAGA NGG 10 8 17 8 24 0 100 5 10
    240606122936_hwen CGGGCTCTCGATAGCACTGCCTTCTCAATAGG(+AAAAAA)GATCCCAGCATCTGTGGCTGTCATTCATAAC NGG 10 8 17 8 24 0 100 5 10
    240606122934_qc4z GGTTGCTCCGTGCATAACTAGTTCTTTCTGGG(+AAAAAA)GATCCTGTCTGTCTCTTTTTTCCCCTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606122933_fhyw GACAGAGGTCAGGGGAAAAAAGAGACAGACAG(+AAAAAA)GATCCCCAGAAAGAACTAGTTATGCACGGAG NGG 10 8 17 8 24 0 100 5 10
    240606122931_qvux AGTGCCTTTTGAGGTCCAGTCGCTGTGGCTCG(+AAAAAA)GATCCATTCATCTCTATGTTACCTCCTACTC NGG 10 8 17 8 24 0 100 5 10
    240606122930_tnkv CAAACGAGTAGGAGGTAACATAGAGATGAATG(+AAAAAA)GATCCGAGCCACAGCGACTGGACCTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606122928_g5ly GAATCTGCTTCATTATTGTCTCGGCTCAGGTG(+AAAAAA)GTACCTGGTGGCTGAGATTTAAGAGTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240606122926_aufw AGCAGGGCCAACTCTTAAATCTCAGCCACCAG(+AAAAAA)GTACCACCTGAGCCGAGACAATAATGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240606122924_2wxz GGCACTCACTAGGAGGACCTGTCCACACTGGG(+AAAAAA)GATCCTGCAGGCCCTGGGTGGGGACAGCACC NGG 10 8 17 8 24 0 100 5 10
    240606122923_wpco GCCAGGGTGCTGTCCCCACCCAGGGCCTGCAG(+AAAAAA)GATCCCCAGTGTGGACAGGTCCTCCTAGTGA NGG 10 8 17 8 24 0 100 5 10