Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123057_nuxl ATAGGCCTGTCACTGCAAAACCACCGTCCCTG(+AAAAAA)GTACCAACTTTTGTCTTAGTTACGATTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606123056_ewgq CAGCAATAAAATCGTAACTAAGACAAAAGTTG(+AAAAAA)GTACCAGGGACGGTGGTTTTGCAGTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606123054_gfkv ATGGGAGAGAGTGACCGAGCTAGGAACAGAGG(+AAAAAA)GATCCCAGCCACTCCAAGGTAGAGTCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606123053_rcjs TCCATACACAGACTCTACCTTGGAGTGGCTGG(+AAAAAA)GATCCCTCTGTTCCTAGCTCGGTCACTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606123051_ojaz CCTGGTGCCCTTGGAGGCAGTAAGAGGGCATG(+AAAAAA)GATCCCCTGGAACTGGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606123049_k4f3 GTTCACAACTGTCTGTAACTCCAGTTCCAGGG(+AAAAAA)GATCCATGCCCTCTTACTGCCTCCAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606123047_v8b1 CTATCTTCAGACACAACCAGAGGAGGGCATTG(+AAAAAA)GATCCTATTACCAATGGTTGTAAGCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240606123046_zltd ACCACATGGTAGCTTACAACCATTGGTAATAG(+AAAAAA)GATCCAATGCCCTCCTCTGGTTGTGTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606123044_54c8 CCCACTCCCACTCCCACTCCCAACCCCCATTG(+AAAAAA)GATCCCTGGATTGTCAGTCAAGAATGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606123042_vj1l TGGGGTTTTTCATTCTTGACTGACAATCCAGG(+AAAAAA)GATCCAATGGGGGTTGGGAGTGGGAGTGGGA NGG 10 8 17 8 24 0 100 5 10