Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123503_idrr TTGTAAAAGGCCTACCTTCAAGACCACCCATG(+AAAAAA)GATCCTCAAACCTATTAATCTGTTAAGGACG NGG 10 8 17 8 24 0 100 5 10
    240606123502_vxwk GGCTCCGTCCTTAACAGATTAATAGGTTTGAG(+AAAAAA)GATCCATGGGTGGTCTTGAAGGTAGGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606123501_lcmx GTCACATTGTATCCAGGCAGGAAGCAGAGATG(+AAAAAA)GATCCAGGTGCTCGGTCCCCTCTCCTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240606123459_4y73 CTATAAGAACAGGAGAGGGGACCGAGCACCTG(+AAAAAA)GATCCATCTCTGCTTCCTGCCTGGATACAAT NGG 10 8 17 8 24 0 100 5 10
    240606123457_jx3h GTAAACACACAAACAGTAAGCTTAGCTGTTGG(+AAAAAA)GATCCTGCCCCGAGGTCACCACTCCCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606123456_q5yu ATGGAATGAAGGGAGTGGTGACCTCGGGGCAG(+AAAAAA)GATCCCAACAGCTAAGCTTACTGTTTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606123454_4rae GTGGGCCTTTTAAATGTCCTATGGGATACAAG(+AAAAAA)GTACCAAGCCAGTGGAACCTGACAGAAATAT NGG 10 8 17 8 24 0 100 5 10
    240606123453_joj0 TTGAAATATTTCTGTCAGGTTCCACTGGCTTG(+AAAAAA)GTACCTTGTATCCCATAGGACATTTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606123452_wfti TGCAGGAGGTTTGTCGCTGAAGCCATGCGATG(+AAAAAA)GATCCAGAGAGACTTTTTAATAATCCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240606123451_2744 TATTACCTAAGGATTATTAAAAAGTCTCTCTG(+AAAAAA)GATCCATCGCATGGCTTCAGCGACAAACCTC NGG 10 8 17 8 24 0 100 5 10