Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123548_448u TGAAGAAGTAGAGAAACAAGTCAGAGTGGTTG(+AAAAAA)GATCCCCTGATTTACTCTGTCAGTACATTTT NGG 10 8 17 8 24 0 100 5 10
    240606123546_2m0a AAAAAAAAATGTACTGACAGAGTAAATCAGGG(+AAAAAA)GATCCAACCACTCTGACTTGTTTCTCTACTT NGG 10 8 17 8 24 0 100 5 10
    240606123545_vp4e GGGGAGACACTATGGCATATATTACAGAGAAG(+AAAAAA)GATCCTTTCTGGCTACTGGTCACTGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240606123543_j1sz TCTTAATGTAGCAGTGACCAGTAGCCAGAAAG(+AAAAAA)GATCCTTCTCTGTAATATATGCCATAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606123542_p4pd GGGATTCTACCGTGCACTGCTGCATCCAGTAG(+AAAAAA)GATCCTAGCTAAAGGCTTTCTCTACTATGAT NGG 10 8 17 8 24 0 100 5 10
    240606123541_fjgo ACAACATCATAGTAGAGAAAGCCTTTAGCTAG(+AAAAAA)GATCCTACTGGATGCAGCAGTGCACGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606123540_qexp AATCTTTTCAGAGAATTCTAAAAAGACTATAG(+AAAAAA)GATCCTATAATAGATCCAATGATCTATAGTG NGG 10 8 17 8 24 0 100 5 10
    240606123539_c61d TATGACACTATAGATCATTGGATCTATTATAG(+AAAAAA)GATCCTATAGTCTTTTTAGAATTCTCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606123537_ue41 GCTCACAACTGTTCGTAACTCAAGTTTCAGAG(+AAAAAA)GATCCAGTGCCCTCTTTGGACTCCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606123536_4zfv CTGGTGCCTGTGGGAGTCCAAAGAGGGCACTG(+AAAAAA)GATCCTCTGAAACTTGAGTTACGAACAGTTG NGG 10 8 17 8 24 0 100 5 10