Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123648_bgy9 AGTGATCGAGGGGGGAGGGCCCCTTGTGCATG(+AAAAAA)GTACCATCTCTAGGCTGGTAGTCTTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606123647_iy90 TTATAGAACTCAAGACTACCAGCCTAGAGATG(+AAAAAA)GTACCATGCACAAGGGGCCCTCCCCCCTCGA NGG 10 8 17 8 24 0 100 5 10
    240606123646_ye25 AGCCCTTGGAGGAGTCTAGAAGATGGTGAGTG(+AAAAAA)GATCCCAGACATTGGACCGGTGGAGTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606123644_mf6g GCAAAATCAAACTCCACCGGTCCAATGTCTGG(+AAAAAA)GATCCACTCACCATCTTCTAGACTCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606123643_b3wf AAAAAATGAGACTTTGACAATTAGTTGGAAAG(+AAAAAA)GATCCAACTGAAATTGTGGTACATTTTACTT NGG 10 8 17 8 24 0 100 5 10
    240606123642_a4fy TGTGTAAGTAAAATGTACCACAATTTCAGTTG(+AAAAAA)GATCCTTTCCAACTAATTGTCAAAGTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606123640_v62d TGAGTGTCTGTTCTGTCCTGTTGGACCTCATG(+AAAAAA)GTACCCACACTTGAGGCAGCTTGTTTCCTTA NGG 10 8 17 8 24 0 100 5 10
    240606123638_7fc5 CTGGGTAAGGAAACAAGCTGCCTCAAGTGTGG(+AAAAAA)GTACCATGAGGTCCAACAGGACAGAACAGAC NGG 10 8 17 8 24 0 100 5 10
    240606123637_hh0c TTGTTAAAGGATTTGAGGGTTTTTCTCTGCAG(+AAAAAA)GTACCACCTGTAAATAAGAAGAAAGTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606123636_9a4u TTTGGCCCCTACTTTCTTCTTATTTACAGGTG(+AAAAAA)GTACCTGCAGAGAAAAACCCTCAAATCCTTT NGG 10 8 17 8 24 0 100 5 10