Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123619_5h3i GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+AAAAAA)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606123617_u7or TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+AAAAAA)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240606123615_ielz TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+AAAAAA)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606123614_q42o GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+AAAAAA)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606123613_n7ny ACAAAGTCCTTTAATTTAAAAAAAAAAAAAAG(+AAAAAA)GTACCTGATTCTCACTTCTCTCAATCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240606123612_5xnj GTAAATAGAAGATTGAGAGAAGTGAGAATCAG(+AAAAAA)GTACCTTTTTTTTTTTTTTAAATTAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606123610_dqzy GTTACAGATTGCTCTTTATTATAATACCAGTG(+AAAAAA)GTACCCAAAGAAAAAAGCAGAGCATTATGTA NGG 10 8 17 8 24 0 100 5 10
    240606123608_iiqj AAACTTACATAATGCTCTGCTTTTTTCTTTGG(+AAAAAA)GTACCACTGGTATTATAATAAAGAGCAATCT NGG 10 8 17 8 24 0 100 5 10
    240606123607_3stv TCACATCACTGGTGTGAAGTGATGTGAAAAGG(+AAAAAA)GTACCTTCATCGAGTGCTGTACCTGCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606123605_uwna GCACAGAACAGCAGGTACAGCACTCGATGAAG(+AAAAAA)GTACCCTTTTCACATCACTTCACACCAGTGA NGG 10 8 17 8 24 0 100 5 10