Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123853_dvsw TTGATGTGGGGGGGCCCAGCCCATTGTGGGTG(+AAAAAA)GTACCATCCTGGGCTGGTGGTCCTGGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240606123852_3i31 CTTATAGAACCCAGGACCACCAGCCCAGGATG(+AAAAAA)GTACCACCCACAATGGGCTGGGCCCCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240606123850_5whj AAGAAAGAAATGCATTAGCCTAAGTCCTTGTG(+AAAAAA)GATCCCAGGCTCCATTCTGCCATTCTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606123848_q6lr AGTAGGAAAGAGAATGGCAGAATGGAGCCTGG(+AAAAAA)GATCCACAAGGACTTAGGCTAATGCATTTCT NGG 10 8 17 8 24 0 100 5 10
    240606123847_dudb ACAAGCAACCGAGTAACAATCTTTCAACACTG(+AAAAAA)GTACCATGAGCAAATCCAGTGTTTTCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606123846_i6cj GCAAATGGAAGAAAACACTGGATTTGCTCATG(+AAAAAA)GTACCAGTGTTGAAAGATTGTTACTCGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606123843_dlpv CACTGCCTCTCAGTAGTTTTAAAATATCCCAG(+AAAAAA)GTACCACGTGCTTACTTTTATTACTTGGGTA NGG 10 8 17 8 24 0 100 5 10
    240606123842_6shl TATATTACCCAAGTAATAAAAGTAAGCACGTG(+AAAAAA)GTACCTGGGATATTTTAAAACTACTGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606123840_c5c4 ACACGTAGTGATGATAGAAATTAGAATTTAAG(+AAAAAA)GATCCTATTGCTATGACTCTCATTTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606123839_a6c7 AGGGCAGAGAGAAATGAGAGTCATAGCAATAG(+AAAAAA)GATCCTTAAATTCTAATTTCTATCATCACTA NGG 10 8 17 8 24 0 100 5 10