Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124100_53vr CTCCTTGCAGGAGGGGATACCACCATTTCAGG(+AAAAAA)GATCCACAATTGAACCTCTGGCTTGATCCCT NGG 10 8 17 8 24 0 100 5 10
    240606124058_jov0 CAGGGAGGGATCAAGCCAGAGGTTCAATTGTG(+AAAAAA)GATCCCTGAAATGGTGGTATCCCCTCCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606124056_apwm GCCATCTACTGAGCGAGTGTGAACTGGCCAGG(+AAAAAA)GATCCACCAGGAAGAGGCATCATCTTCATTA NGG 10 8 17 8 24 0 100 5 10
    240606124055_xtgi CAATATAATGAAGATGATGCCTCTTCCTGGTG(+AAAAAA)GATCCCTGGCCAGTTCACACTCGCTCAGTAG NGG 10 8 17 8 24 0 100 5 10
    240606124054_wc2j ACCATGGAATGGATGCCCTGGTGTATTGTCTG(+AAAAAA)GATCCCCAGAAATACTTATCACCCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606124052_yew1 GTCCAGTGGCTGGGGTGATAAGTATTTCTGGG(+AAAAAA)GATCCAGACAATACACCAGGGCATCCATTCC NGG 10 8 17 8 24 0 100 5 10
    240606124050_7h8d CAGAGGCACATCCTTTTCCGTGGCAGCATCAG(+AAAAAA)GATCCACTCATGTTGCTGCTGGATGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606124049_xjb5 GCTCTGCCCTCCATCCAGCAGCAACATGAGTG(+AAAAAA)GATCCTGATGCTGCCACGGAAAAGGATGTGC NGG 10 8 17 8 24 0 100 5 10
    240606124048_jlvh AACCTTGTGAGCAGCCAATCCAGTTCTAGCTG(+AAAAAA)GATCCCTCCACCCCACCCCCACCCCACCCCA NGG 10 8 17 8 24 0 100 5 10
    240606124046_knce TGGGGTGGGGTGGGGTGGGGGTGGGGTGGAGG(+AAAAAA)GATCCAGCTAGAACTGGATTGGCTGCTCACA NGG 10 8 17 8 24 0 100 5 10