Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124148_hf0b CCGGAGGAAACATGAGCGGGATCGTCTGAGCG(+AAAAAA)GATCCCTTTCCCTGGCTGTGTGCGCGTGCGT NGG 10 8 17 8 24 0 100 5 10
    240606124146_gtwo ACTCCACGCACGCGCACACAGCCAGGGAAAGG(+AAAAAA)GATCCGCTCAGACGATCCCGCTCATGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606124145_z71s AGGAACTCAGGCTGACCTCAGACTCCATGCAG(+AAAAAA)GATCCTCCTGTCAGAGCCATGATGACATAAA NGG 10 8 17 8 24 0 100 5 10
    240606124143_rs6e GTTGCTTTATGTCATCATGGCTCTGACAGGAG(+AAAAAA)GATCCTGCATGGAGTCTGAGGTCAGCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606124142_5pft CTGAGAACTTGAGACAAGGCTTACCACAGGAG(+AAAAAA)GTACCCACTCCCCCATACCTCCTGGACAAGC NGG 10 8 17 8 24 0 100 5 10
    240606124140_izqo TGTCTGCTTGTCCAGGAGGTATGGGGGAGTGG(+AAAAAA)GTACCTCCTGTGGTAAGCCTTGTCTCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606124139_0nu9 GAATCAGATAACAGAATCAGTGTTTCAGTCAG(+AAAAAA)GATCCACACTGCCTGCCAGGCTGTGCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606124137_3883 CACATCCACAGCACAGCCTGGCAGGCAGTGTG(+AAAAAA)GATCCTGACTGAAACACTGATTCTGTTATCT NGG 10 8 17 8 24 0 100 5 10
    240606124136_l7t2 CGGTGAAGCCTTACGACTCCACCACAGTTCGG(+AAAAAA)GATCCTAGATTCTGGCGTACATAGCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606124134_k1wj AGAGTTGCAGAGCTATGTACGCCAGAATCTAG(+AAAAAA)GATCCCGAACTGTGGTGGAGTCGTAAGGCTT NGG 10 8 17 8 24 0 100 5 10