Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124316_t7dz CTTGCAGGTGCCCTTGGAGGCCAGGAACGTTG(+AAAAAA)GATCCCCTGGGACCTGATATTACAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606124313_dhc2 CTGACAGCCACCTGTAATATCAGGTCCCAGGG(+AAAAAA)GATCCAACGTTCCTGGCCTCCAAGGGCACCT NGG 10 8 17 8 24 0 100 5 10
    240606124312_4o1s CCCCAGGTGCCTAAATTCCAGGAGAATCACAG(+AAAAAA)GTACCAGTCTCAATTCACCACTTAACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240606124311_8f15 ACTTCCTAAAGTTAAGTGGTGAATTGAGACTG(+AAAAAA)GTACCTGTGATTCTCCTGGAATTTAGGCACC NGG 10 8 17 8 24 0 100 5 10
    240606124309_fkua CCATCCTGGGAAAGTGACTGGGGCCCACGTGG(+AAAAAA)GATCCTAGAGCCTAGCACAGTGATATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240606124308_6diz TGCTTTGGACATATCACTGTGCTAGGCTCTAG(+AAAAAA)GATCCCACGTGGGCCCCAGTCACTTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606124307_5akk TGTACTTCCTTCATCCGGTGGGTTCCCTGCTG(+AAAAAA)GTACCACTCTCCAGAATCCATACATGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606124305_usr9 ATCAACACACCATGTATGGATTCTGGAGAGTG(+AAAAAA)GTACCAGCAGGGAACCCACCGGATGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606124304_3jnm GTTTCTAGATGAAAGGGGATTTCTATTCAGAG(+AAAAAA)GTACCTGTGTGTATCTGTTAGTAACCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606124303_ez8h TAGTCCCTGGGGTTACTAACAGATACACACAG(+AAAAAA)GTACCTCTGAATAGAAATCCCCTTTCATCTA NGG 10 8 17 8 24 0 100 5 10