Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124556_n9ze TAAAATCTGAGGTAGGGAAAGGTAAAAAGCAG(+AAAAAA)GTACCCTCTCTTCAAATAGAAGCGCATGTTC NGG 10 8 17 8 24 0 100 5 10
    240606124554_c5z4 ACAAGGAACATGCGCTTCTATTTGAAGAGAGG(+AAAAAA)GTACCTGCTTTTTACCTTTCCCTACCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606124553_3yrq AGTACTCACATTGCTACTGTCTTCTGTTGTAG(+AAAAAA)GTACCATGCTCGGAGCTTGCTTACAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606124552_2nu6 AAATTTTTATTTGTAAGCAAGCTCCGAGCATG(+AAAAAA)GTACCTACAACAGAAGACAGTAGCAATGTGA NGG 10 8 17 8 24 0 100 5 10
    240606124551_9ng0 GACATTTTTACATTAGCAAAGTCACCTCATGG(+AAAAAA)GATCCTGCAGTTAAAGTGTTAAGACGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606124549_cdmj TCTGCAGCCTCGTCTTAACACTTTAACTGCAG(+AAAAAA)GATCCCATGAGGTGACTTTGCTAATGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606124548_xror GTCACTGAGAACTTTGAGCACCCTGGGACGAG(+AAAAAA)GTACCCCCACCTCAATGATATTTCTCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606124546_c0np GTTGTTCTCAGAGAAATATCATTGAGGTGGGG(+AAAAAA)GTACCTCGTCCCAGGGTGCTCAAAGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606124545_jt92 TTCTAATTCCTGGAAATTGTGTGGGTGGAGTG(+AAAAAA)GATCCCTGCACTGGGGGGAATAACATGGACC NGG 10 8 17 8 24 0 100 5 10
    240606124543_qlws TCAGTGGTCCATGTTATTCCCCCCAGTGCAGG(+AAAAAA)GATCCACTCCACCCACACAATTTCCAGGAAT NGG 10 8 17 8 24 0 100 5 10