Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124611_2dan CCAAGAGGATGGGATTCATATTTAGTCCCTAG(+AAAAAA)GTACCGTTAAGAAGTGGCACACTATTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240606124610_9hjd ATATACTTACAATAGTGTGCCACTTCTTAACG(+AAAAAA)GTACCTAGGGACTAAATATGAATCCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240606124608_v3pa TCATGCAGTGTCACCCGTGTCACGCAGCGCGG(+AAAAAA)GTACCCTGAGAGGACTCTGAATACCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606124606_8shw AGTTGGGGAAGGGTATTCAGAGTCCTCTCAGG(+AAAAAA)GTACCCGCGCTGCGTGACACGGGTGACACTG NGG 10 8 17 8 24 0 100 5 10
    240606124604_d0qy TCATTTTGTTTCCTTTTAAGAAATTGCCACAG(+AAAAAA)GTACCCCTACGTTTAGTAACTACCATGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606124603_7ip8 ACTTATCAGCATGGTAGTTACTAAACGTAGGG(+AAAAAA)GTACCTGTGGCAATTTCTTAAAAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606124601_b023 TATATTTCCCTTTCCCATTCAATCAATAGGTG(+AAAAAA)GATCCAAGCTTGTTAGCTACTCAGGACTTGC NGG 10 8 17 8 24 0 100 5 10
    240606124600_inza CTACTGCAAGTCCTGAGTAGCTAACAAGCTTG(+AAAAAA)GATCCACCTATTGATTGAATGGGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606124558_xhb0 CAAGTTCCAAGCCAACCAGGGGTACAAAATGG(+AAAAAA)GATCCTGACTTTAAAATTGAACAATTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606124557_9a5a GTCATTTTTAAATTGTTCAATTTTAAAGTCAG(+AAAAAA)GATCCCATTTTGTACCCCTGGTTGGCTTGGA NGG 10 8 17 8 24 0 100 5 10