Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124714_h8ya CTGTGTATCTTTAAATACATCTGGATTGTGGG(+AAAAAA)GTACCTATGCGCAGTCTAGTTCAAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606124713_ufq3 ATTTATTTATTTTTGAACTAGACTGCGCATAG(+AAAAAA)GTACCCCACAATCCAGATGTATTTAAAGATA NGG 10 8 17 8 24 0 100 5 10
    240606124712_tmq2 ACACTGTGCCCTAGTTCTGTGAGAATGAGCAG(+AAAAAA)GTACCTCTCTTCCCAGAGGCCATCACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606124710_qnq9 TCCCATCTAGGTGATGGCCTCTGGGAAGAGAG(+AAAAAA)GTACCTGCTCATTCTCACAGAACTAGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606124708_djpp GGATTTCAAAGTGATGTCCGATGTAGCGCCAG(+AAAAAA)GTACCTTTGAATATCAATCAGAACCATTTAG NGG 10 8 17 8 24 0 100 5 10
    240606124707_zvtj CATATCTAAATGGTTCTGATTGATATTCAAAG(+AAAAAA)GTACCTGGCGCTACATCGGACATCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606124706_n1ib TGTTTCGGGCAGTGCTCTCCCTAGTTTAAAAG(+AAAAAA)GTACCCAGGATCTTGTGTGGCTTGGATTTAA NGG 10 8 17 8 24 0 100 5 10
    240606124703_d8mh GGCATTTAAATCCAAGCCACACAAGATCCTGG(+AAAAAA)GTACCTTTTAAACTAGGGAGAGCACTGCCCG NGG 10 8 17 8 24 0 100 5 10
    240606124702_nzmc GAGTGAAAACAAGCTTTTCTTCAAATCTTCAG(+AAAAAA)GTACCTACCTCAGAATAGGTGTCATTAACGT NGG 10 8 17 8 24 0 100 5 10
    240606124700_4pgi TGAAAACGTTAATGACACCTATTCTGAGGTAG(+AAAAAA)GTACCTGAAGATTTGAAGAAAAGCTTGTTTT NGG 10 8 17 8 24 0 100 5 10