Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124800_iqwe TCCCATTAAGGAAAATGTGAAAATTGCATATG(+AAAAAA)GATCCACTCTCACCATAAAGGTGAGAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606124758_3cu3 CCTGCCTTCTTCTCACCTTTATGGTGAGAGTG(+AAAAAA)GATCCATATGCAATTTTCACATTTTCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606124757_2l32 AGTGTCTGGGTTTGCTGTCTGCAAGTGGAATG(+AAAAAA)GATCCCAAGGTGGGCCAGTGTCTTGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240606124755_zqvl GGAAAGGCCATCAAGACACTGGCCCACCTTGG(+AAAAAA)GATCCATTCCACTTGCAGACAGCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606124753_vqtq GAAAAACTGTTTATAAGAACCAAAATGATGTG(+AAAAAA)GATCCCTGTGTGTCCTGTGGAGGTGACATCA NGG 10 8 17 8 24 0 100 5 10
    240606124752_cnjj GAACTTGATGTCACCTCCACAGGACACACAGG(+AAAAAA)GATCCACATCATTTTGGTTCTTATAAACAGT NGG 10 8 17 8 24 0 100 5 10
    240606124751_c191 ATCAACAGCAAACTTAAAGGAAAAAGGAGCTG(+AAAAAA)GTACCCCAATGTCCTGACCTGGAGTCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606124749_85wr GCCAAATCTTGACTCCAGGTCAGGACATTGGG(+AAAAAA)GTACCAGCTCCTTTTTCCTTTAAGTTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606124747_36fe GCTACCTTTTAGGACTGAAGTTTTAAGCACTG(+AAAAAA)GTACCCACTGTGGGGCAAACCTGTGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606124746_9lbk CCCTCCTGCAGCACAGGTTTGCCCCACAGTGG(+AAAAAA)GTACCAGTGCTTAAAACTTCAGTCCTAAAAG NGG 10 8 17 8 24 0 100 5 10