Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130034_v0xr AGAAAGCACCAGGTCAGGCAGGGCCTGAGGTG(+AAAAAA)GATCCGAGCTGGGTCCAGGGCTCGAATCTCA NGG 10 8 17 8 24 0 100 5 10
    240606130033_w6te TGGTGTGAGATTCGAGCCCTGGACCCAGCTCG(+AAAAAA)GATCCACCTCAGGCCCTGCCTGACCTGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606130031_capb GAGACCCCGAGGGAGGTGAGCTTGAAGTGAGG(+AAAAAA)GTACCATCCAAGGTACTCCAGGACCCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606130030_2g67 GACCAGGAGAGGGTCCTGGAGTACCTTGGATG(+AAAAAA)GTACCCTCACTTCAAGCTCACCTCCCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240606130028_sl0w GGACCGGCCTTTACTGAGCTGTCACACAGTGG(+AAAAAA)GTACCTCTAGGTGCTTTTGGGATGGCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240606130027_2qe6 AGAGATACCTGCCATCCCAAAAGCACCTAGAG(+AAAAAA)GTACCCACTGTGTGACAGCTCAGTAAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606130026_j8on CAGGGGATTTCATCAAGAATCCCAACTATTTG(+AAAAAA)GTACCAGAGCCAAGCAAATGCAAGCAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606130025_p6ol CCAAGTGTTCTGCTTGCATTTGCTTGGCTCTG(+AAAAAA)GTACCAAATAGTTGGGATTCTTGATGAAATC NGG 10 8 17 8 24 0 100 5 10
    240606130023_8ywi GAGCTGCTGCCACACTCGGGCCCAGCGAGGAG(+AAAAAA)GATCCGGGAAAGGCACCTCGGAACTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606130021_5f33 CTTTGTGGCTGAGTTCCGAGGTGCCTTTCCCG(+AAAAAA)GATCCTCCTCGCTGGGCCCGAGTGTGGCAGC NGG 10 8 17 8 24 0 100 5 10