Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130140_r7va TGGGGCGCACATCCCACCTCAGTGTTCTAAAG(+AAAAAA)GATCCAGGGCCACTTTGTCTTCAAGTGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606130138_h0os CACAAATGACACTTGAAGACAAAGTGGCCCTG(+AAAAAA)GATCCTTTAGAACACTGAGGTGGGATGTGCG NGG 10 8 17 8 24 0 100 5 10
    240606130136_04xg AACCCTCTTGGAATCATGATTTGTATTTTATG(+AAAAAA)GTACCTTCTCTCTAAACTCATAGATTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606130135_gq4p AACAAAAAACAATCTATGAGTTTAGAGAGAAG(+AAAAAA)GTACCATAAAATACAAATCATGATTCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606130134_z9eg CATAGTAAAGGGATGTATTTGTTAGTCCTAAG(+AAAAAA)GTACCTAAGTTACAGCTGACCACCAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606130132_difc ACATCTGTTCTTGGTGGTCAGCTGTAACTTAG(+AAAAAA)GTACCTTAGGACTAACAAATACATCCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606130131_f7ma CTTTGCTACAACCCAATGCTATAACTGTGGTG(+AAAAAA)GATCCTATGTTTATAATCTGGAGACACAACA NGG 10 8 17 8 24 0 100 5 10
    240606130130_jdzd AGCAATGTTGTGTCTCCAGATTATAAACATAG(+AAAAAA)GATCCACCACAGTTATAGCATTGGGTTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606130127_deun GGTCACAGCCATCCAAAACTCCAGTTCCAGGG(+AAAAAA)GATCCAATGCCCACATCTGAACTCTATAGGC NGG 10 8 17 8 24 0 100 5 10
    240606130126_6r4l TTGGTGCCTATAGAGTTCAGATGTGGGCATTG(+AAAAAA)GATCCCCTGGAACTGGAGTTTTGGATGGCTG NGG 10 8 17 8 24 0 100 5 10