Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124919_9m45 GCGAGGCCGCAGTCGCCCTCGGGGTTCTTAGG(+AAAAAA)GATCCCCAGCCTCTTGACAACAAGGCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606124917_zufh GGGCCTGCCCGCCTTGTTGTCAAGAGGCTGGG(+AAAAAA)GATCCCTAAGAACCCCGAGGGCGACTGCGGC NGG 10 8 17 8 24 0 100 5 10
    240606124916_ga1n GAAGAGCAGCTTGCCACAGCTGGCCTACAATG(+AAAAAA)GTACCAGCATTCTCTTCGTGATCTCAACACA NGG 10 8 17 8 24 0 100 5 10
    240606124915_72fo CACAGTGTGTTGAGATCACGAAGAGAATGCTG(+AAAAAA)GTACCATTGTAGGCCAGCTGTGGCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606124913_hg7j GCACAGACCTAGGGATGCACTCCCTGCCTTTG(+AAAAAA)GATCCCCACTCTACCATTTGCTAATTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606124911_qxuq AAGGTCAACTAATTAGCAAATGGTAGAGTGGG(+AAAAAA)GATCCAAAGGCAGGGAGTGCATCCCTAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606124910_xt5g TCAAATCCAAAGCCGGCCTGTGTGATGTAGTG(+AAAAAA)GTACCTCTTTCCATCTCAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606124909_tly8 GACTTTTTTTTTTTTTTTTGAGATGGAAAGAG(+AAAAAA)GTACCACTACATCACACAGGCCGGCTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606124907_sl4e GAGGAGAGAAGACACTGGGGGCCTGGAGCTGG(+AAAAAA)GTACCAGGGCTACTACTGCCTTCCACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606124905_gx42 TGGTCTCACAGTGGAAGGCAGTAGTAGCCCTG(+AAAAAA)GTACCCAGCTCCAGGCCCCCAGTGTCTTCTC NGG 10 8 17 8 24 0 100 5 10