Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125024_2m5w TCCCCAGGCCCTTGATAACTTTTCCCCTTCAG(+AAAAAA)GATCCCACTCATAGTAGATTTATTTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606125022_tw0p AATCCTACTTAAAATAAATCTACTATGAGTGG(+AAAAAA)GATCCTGAAGGGGAAAAGTTATCAAGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606125020_fob5 CTTGTACTTCTGTTACTCTAGAACCACTCCTG(+AAAAAA)GTACCAACTTCTGTATTAGTCAGGGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606125019_cs52 ACTCTAGAGAACCCTGACTAATACAGAAGTTG(+AAAAAA)GTACCAGGAGTGGTTCTAGAGTAACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606125017_cyj3 TTCTTTAAAGTTCTGGAGAATGGGTGAAGCTG(+AAAAAA)GTACCTTGCAGAGAAGGGACACAAGGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606125016_8g8x GTGGCATCTTCCTTGTGTCCCTTCTCTGCAAG(+AAAAAA)GTACCAGCTTCACCCATTCTCCAGAACTTTA NGG 10 8 17 8 24 0 100 5 10
    240606125015_droy AAAAACTTCAGAGTTGCAGACAGGACAGTCAG(+AAAAAA)GTACCCCATGGGAGTTCCTAGGGCCTGCGTA NGG 10 8 17 8 24 0 100 5 10
    240606125013_oxm7 ATCCATACGCAGGCCCTAGGAACTCCCATGGG(+AAAAAA)GTACCTGACTGTCCTGTCTGCAACTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606125012_hqra ATGCGCTCTGCACTGCAGCCCCATCTATTCAG(+AAAAAA)GATCCTGCCACCCATGAGGAGATGCCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606125010_4gwg TCCCGTGCTGGGCATCTCCTCATGGGTGGCAG(+AAAAAA)GATCCTGAATAGATGGGGCTGCAGTGCAGAG NGG 10 8 17 8 24 0 100 5 10