Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125009_ppyi GCTGCAGCCCCTGCAGCCCCAACCGCAAGCTG(+AAAAAA)GATCCGGGATCTGCACATCAGCAGCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240606125006_xqsc GGGCTCCAAAGGCTGCTGATGTGCAGATCCCG(+AAAAAA)GATCCAGCTTGCGGTTGGGGCTGCAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606125005_s7rr ACACTAAATAAAACCAAGCCAATAGTCTTGTG(+AAAAAA)GATCCCTGAATATTTCTCATTCCACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240606125004_banv TAAGATGAAGTGTGGAATGAGAAATATTCAGG(+AAAAAA)GATCCACAAGACTATTGGCTTGGTTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240606125002_st3z CTTGCCTGTCTGGTCAGCAGTATTGCACACAG(+AAAAAA)GATCCACAGATGACAGTTGATGGCTCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606125001_aelt GGTGGGGGGAGAGCCATCAACTGTCATCTGTG(+AAAAAA)GATCCTGTGTGCAATACTGCTGACCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240606125000_33hr TCTTAGGAGAGCAAGGATATGGAGTCATTGTG(+AAAAAA)GATCCCTGCCTGTGGGGGGTTTGTGAGATAA NGG 10 8 17 8 24 0 100 5 10
    240606124958_h55g TGCTTTTATCTCACAAACCCCCCACAGGCAGG(+AAAAAA)GATCCACAATGACTCCATATCCTTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606124957_llou ACATGCTGGGAGAAAATTAACACTAAAATCTG(+AAAAAA)GTACCAGCCAGAGGAAGGAACTTGACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240606124955_tpjd CTTATTTTGAGTCAAGTTCCTTCCTCTGGCTG(+AAAAAA)GTACCAGATTTTAGTGTTAATTTTCTCCCAG NGG 10 8 17 8 24 0 100 5 10