Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124954_9tki GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+AAAAAA)GATCCCCAGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606124952_febv GGATGGACCATCTAGAGACTGCCATATCTGGG(+AAAAAA)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606124951_7944 AGTGTTGGCCTATAGGCTGTACAAATATCAAG(+AAAAAA)GTACCCGGAGTAGTTACTCCTCTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606124948_m1fa TTTTTTCTGAAGAAGAGGAGTAACTACTCCGG(+AAAAAA)GTACCTTGATATTTGTACAGCCTATAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606124947_hty5 AAGATGGGGCTCAAGAAATGGTCAGCTCTTGG(+AAAAAA)GTACCTAAGTATTTGAACAGAAGCTGCCACA NGG 10 8 17 8 24 0 100 5 10
    240606124946_9evw GCTTTTGTGGCAGCTTCTGTTCAAATACTTAG(+AAAAAA)GTACCCAAGAGCTGACCATTTCTTGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606124944_d8tg CAAGCCTTCGGCAGAGGGTCATGTACCACTGG(+AAAAAA)GATCCCAAGTACGGCTAGACCCACCAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606124942_8p4e TTGGCCTTTTTGGTGGGTCTAGCCGTACTTGG(+AAAAAA)GATCCCAGTGGTACATGACCCTCTGCCGAAG NGG 10 8 17 8 24 0 100 5 10
    240606124941_nymc ATAAAGTTCAGTGCTATAGCACTTTGTGTGAG(+AAAAAA)GTACCTGGGTTCAACCCCAGCACTGCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606124938_zs3w GTGGGATGAGGCAGTGCTGGGGTTGAACCCAG(+AAAAAA)GTACCTCACACAAAGTGCTATAGCACTGAAC NGG 10 8 17 8 24 0 100 5 10