Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124831_evtc GGAACCCCTTGCACTGTGAAGCTAGAGCGTGG(+AAAAAA)GTACCTAACTCACCGTTGTCCCAGAATGAGG NGG 10 8 17 8 24 0 100 5 10
    240606124830_gaob CCCCGCCTCATTCTGGGACAACGGTGAGTTAG(+AAAAAA)GTACCCACGCTCTAGCTTCACAGTGCAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606124826_s82x TCTGCGTGGGAGAACTTGGTGTCTCACCCTGG(+AAAAAA)GTACCCATTCCTGGTGTATGGAAGCACCTCC NGG 10 8 17 8 24 0 100 5 10
    240606124824_bxr3 GTGAAGGAGGTGCTTCCATACACCAGGAATGG(+AAAAAA)GTACCCAGGGTGAGACACCAAGTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240606124823_v1pr ATGCAGATCAGTTCTGCACACCTCCAGGGGTG(+AAAAAA)GATCCAGCTGTAAGGCCATACCTATATCTTC NGG 10 8 17 8 24 0 100 5 10
    240606124821_tnzs ATCTGGAAGATATAGGTATGGCCTTACAGCTG(+AAAAAA)GATCCACCCCTGGAGGTGTGCAGAACTGATC NGG 10 8 17 8 24 0 100 5 10
    240606124820_urta CACTGATTGAGAAGGAGAGTGTATAATTTAAG(+AAAAAA)GTACCTTAAGTTTAATCTTTGAAAGATTGCC NGG 10 8 17 8 24 0 100 5 10
    240606124819_joq8 AAGATGGCAATCTTTCAAAGATTAAACTTAAG(+AAAAAA)GTACCTTAAATTATACACTCTCCTTCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240606124818_sa22 GAAAACAGCATCAGAGTTCACTTCGCATCATG(+AAAAAA)GTACCCCAAGGGATGGGGATACGGGTATGAC NGG 10 8 17 8 24 0 100 5 10
    240606124815_itaj TCAGTGTCATACCCGTATCCCCATCCCTTGGG(+AAAAAA)GTACCATGATGCGAAGTGAACTCTGATGCTG NGG 10 8 17 8 24 0 100 5 10