Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124744_voqv GGTGAGCTCTGTACAGTAATGGTGGTGTCCAG(+AAAAAA)GTACCGCTAGGAAACACGGGAGTCCGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606124743_1zas AGCCGCTGGACGGACTCCCGTGTTTCCTAGCG(+AAAAAA)GTACCTGGACACCACCATTACTGTACAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606124741_9r10 GAAGCCCCGTGGAAGCCAAAAGCAGTTTGGTG(+AAAAAA)GATCCGTGGCAGCAGCTTGGAGCTGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240606124739_x346 TTTTGAAATCACAGCTCCAAGCTGCTGCCACG(+AAAAAA)GATCCACCAAACTGCTTTTGGCTTCCACGGG NGG 10 8 17 8 24 0 100 5 10
    240606124737_gt5o GAGGGACCATTTTCCTGACTGTACCTCTTTGG(+AAAAAA)GTACCCCATTCCTAAAGCAATAACTCAACGG NGG 10 8 17 8 24 0 100 5 10
    240606124735_itjs CATTACCGTTGAGTTATTGCTTTAGGAATGGG(+AAAAAA)GTACCCAAAGAGGTACAGTCAGGAAAATGGT NGG 10 8 17 8 24 0 100 5 10
    240606124734_s59q CGTCCCTAGAAGCACAGGGCCCCTAGAAGCAG(+AAAAAA)GTACCAGCTCTGAGAACTTCCTGGCTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606124733_pefg TGCACCAAGGAGCCAGGAAGTTCTCAGAGCTG(+AAAAAA)GTACCTGCTTCTAGGGGCCCTGTGCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240606124732_xv24 TTCTCATTTGGGCTGGGGTGCCTATTTGTTTG(+AAAAAA)GTACCCCTCTGCTAAGTCACAAAGCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606124730_sx9f TACCCACAAAAGCTTTGTGACTTAGCAGAGGG(+AAAAAA)GTACCAAACAAATAGGCACCCCAGCCCAAAT NGG 10 8 17 8 24 0 100 5 10