Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130021_5f33 CTTTGTGGCTGAGTTCCGAGGTGCCTTTCCCG(+AAAAAA)GATCCTCCTCGCTGGGCCCGAGTGTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606130019_ugot GTCTTCTCCCAATCACAGTTCCAGGGGCCAGG(+AAAAAA)GTACCTCCAGAGGACTTCTCAAGCTGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606130017_4lh5 GAAACCCAACCAGCTTGAGAAGTCCTCTGGAG(+AAAAAA)GTACCCTGGCCCCTGGAACTGTGATTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606130016_8yiz TATACTTGGGGACAGTTACAAGGGAGTCACAG(+AAAAAA)GTACCCTGTCCATGTCTGCGGCTGTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606130014_olcz GCAGGCTGCCCACAGCCGCAGACATGGACAGG(+AAAAAA)GTACCTGTGACTCCCTTGTAACTGTCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240606130013_vr70 CAAGTCAGAATTCCTGGTAGGGGCGGTTTCAG(+AAAAAA)GATCCCACAGAAATGAAGGGACACAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606130011_3vus TGGGAGAAGACTGTGTCCCTTCATTTCTGTGG(+AAAAAA)GATCCTGAAACCGCCCCTACCAGGAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240606130009_bsrs CATCCAGGCCCCCAATCTTTTATATTGAAGGG(+AAAAAA)GATCCAACTTCCAGGATGAAAGGCAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606130008_rs5q ACCAGGCTCTCTGCCTTTCATCCTGGAAGTTG(+AAAAAA)GATCCCCTTCAATATAAAAGATTGGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606130007_5i2w GCTGAGCAAGAAAGGCTGTCTGAGCCATGGTG(+AAAAAA)GATCCCCAGGGAGGGAATGTCCCCTCCCCCC NGG 10 8 17 8 24 0 100 5 10