Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124728_2q6r TTTGGTTTTTGCTGCTTGAGACCTTCTTTTGG(+AAAAAA)GTACCATTCACAGAGGACACTGAGGTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606124727_llh8 GAAGCCACCAACCTCAGTGTCCTCTGTGAATG(+AAAAAA)GTACCCAAAAGAAGGTCTCAAGCAGCAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606124725_l15j TAAGTTCCCCTTAGCCTTGTGAGCCTTACTGG(+AAAAAA)GATCCAGTCCCCATATAGCATCCCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240606124724_hal5 AAGGAAGTTGGAGGGATGCTATATGGGGACTG(+AAAAAA)GATCCCAGTAAGGCTCACAAGGCTAAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606124723_3jwx ACTGAATACAGAACAGCGTGACTAGAAAGTTG(+AAAAAA)GATCCTGCGCTCTTGGACTGTTGGGAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606124721_296z TTTTTTCCTTTCCCAACAGTCCAAGAGCGCAG(+AAAAAA)GATCCAACTTTCTAGTCACGCTGTTCTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606124719_1lpj GGAAATATGAGAGGACAGAAACTAAAAAATTG(+AAAAAA)GATCCAGTTCCAAGACAACTATTATCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606124718_fu72 ACAGCATCAAGATAATAGTTGTCTTGGAACTG(+AAAAAA)GATCCAATTTTTTAGTTTCTGTCCTCTCATA NGG 10 8 17 8 24 0 100 5 10
    240606124717_7225 CCCTATGTCTCCACTCGCAATCATATACTTAG(+AAAAAA)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240606124716_u1yq GAATGTTAGATATTTTTTCCATGGGGTCAAAG(+AAAAAA)GTACCTAAGTATATGATTGCGAGTGGAGACA NGG 10 8 17 8 24 0 100 5 10