Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124625_ov0k TGCGTTCCTGCAGTGTCCTCCTCGAGGAGCAG(+AAAAAA)GATCCACATTTTCAAGAATGTCCTGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606124624_p1nq TCACGAGGAGTCAGGACATTCTTGAAAATGTG(+AAAAAA)GATCCTGCTCCTCGAGGAGGACACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606124623_p48g AGCACATGTCAAGAAGGACTCAGGAAAGAAAG(+AAAAAA)GTACCTGTGCCTTGCCCCAGTGTCTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606124622_7ju0 TGAACTCAGACAGACACTGGGGCAAGGCACAG(+AAAAAA)GTACCTTTCTTTCCTGAGTCCTTCTTGACAT NGG 10 8 17 8 24 0 100 5 10
    240606124621_zhrv ATACCTTCCAAGGACCAGTAGACTTGAATACG(+AAAAAA)GATCCAATGGGAGTTGGTTGTCTGGTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606124618_7z6r TCATCTTGACACCAGACAACCAACTCCCATTG(+AAAAAA)GATCCGTATTCAAGTCTACTGGTCCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606124617_ydov GACTCCCTCCAGGGGTTCCCAGCTCCCACAGG(+AAAAAA)GTACCTGCCATCTCCATCCCTGGACTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606124616_yv1m GTGGCTCTGCAGTCCAGGGATGGAGATGGCAG(+AAAAAA)GTACCCTGTGGGAGCTGGGAACCCCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606124614_auvn ATTTTGGAAGAACACAGAAAGTTTTCAGACAG(+AAAAAA)GATCCATTTCCCCAGTACTCAACCAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240606124613_62q7 CTTTCCAATTTTGGTTGAGTACTGGGGAAATG(+AAAAAA)GATCCTGTCTGAAAACTTTCTGTGTTCTTCC NGG 10 8 17 8 24 0 100 5 10