Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122112_cayx ATGGTATATAAAGAGTGTACCTTATAAGCCAG(+AAAAAA)GTACCTTTAATCCCAGAACTTGGGTTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606122111_qytl GCTGGCTTCAAACCCAAGTTCTGGGATTAAAG(+AAAAAA)GTACCTGGCTTATAAGGTACACTCTTTATAT NGG 10 8 17 8 24 0 100 5 10
    240606122109_bxz5 GAATAAAAGAATAATATATAAAATTAATAAAG(+AAAAAA)GTACCAAATGCATCTATAAAAAATGGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606122108_npu7 TCTCAAGGAACCATTTTTTATAGATGCATTTG(+AAAAAA)GTACCTTTATTAATTTTATATATTATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606122107_mtvl ATCTGTTCTATGGAATAAGAAAGGGAGGCGTG(+AAAAAA)GTACCCAAAGCATCCTGTAAAGCCAGCATTA NGG 10 8 17 8 24 0 100 5 10
    240606122105_0jda TAGGGTAATGCTGGCTTTACAGGATGCTTTGG(+AAAAAA)GTACCACGCCTCCCTTTCTTATTCCATAGAA NGG 10 8 17 8 24 0 100 5 10
    240606122104_v7jt AAAAGAATATACTTTCTTCTCAGTGCCTCATG(+AAAAAA)GTACCATCTCCAAAATTAACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240606122103_kl7q TGTGACCAATTATATGGTTAATTTTGGAGATG(+AAAAAA)GTACCATGAGGCACTGAGAAGAAAGTATATT NGG 10 8 17 8 24 0 100 5 10
    240606122101_sj94 AGATTATTGCCACACATTGTAGGGAGGCAGAG(+AAAAAA)GTACCATGCCTTGCCCTGGGTATGCCTTGCC NGG 10 8 17 8 24 0 100 5 10
    240606122100_3kt6 GTGGTGGCAAGGCATACCCAGGGCAAGGCATG(+AAAAAA)GTACCTCTGCCTCCCTACAATGTGTGGCAAT NGG 10 8 17 8 24 0 100 5 10