Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122013_pn7k AAGAATAAGAAACCAAGAGTCGAGAGATCCAG(+AAAAAA)GATCCCATTCTGCGGCTGCTGTTTGCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240606122011_typj GGGTTACTCAGCAAACAGCAGCCGCAGAATGG(+AAAAAA)GATCCTGGATCTCTCGACTCTTGGTTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240606122009_me6r CCTTGTGCTCTGTCAGGAGCCATAGCTCCTGG(+AAAAAA)GATCCTTAAGATAGAAGTGGGTGACCCGTGT NGG 10 8 17 8 24 0 100 5 10
    240606122007_dqdn AGTGTACACGGGTCACCCACTTCTATCTTAAG(+AAAAAA)GATCCCAGGAGCTATGGCTCCTGACAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606122006_04xg AATCTTCTCTACCCAAATCCTCCTCATAAATG(+AAAAAA)GATCCATGATGACAGCTAAGAGAAAAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606122005_ijiu TACTTATTTTTTTTCTCTTAGCTGTCATCATG(+AAAAAA)GATCCATTTATGAGGAGGATTTGGGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606122003_pvus TGCAAAGGACTGGAGATACAGGGTTAAGACAG(+AAAAAA)GTACCATCTACATATATTCTGAACAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240606122002_fikq CTACACTGCATTGTTCAGAATATATGTAGATG(+AAAAAA)GTACCTGTCTTAACCCTGTATCTCCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606122000_ghif GGGTAATGCAAAGAAAATGAGTTTTCCTTTGG(+AAAAAA)GTACCAGTAATTGTCAAAGGAATGATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606121959_bsr9 TGAATCTGCAATCATTCCTTTGACAATTACTG(+AAAAAA)GTACCCAAAGGAAAACTCATTTTCTTTGCAT NGG 10 8 17 8 24 0 100 5 10