Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121926_ntkk CAAAATGTTTGATAATTATTCCAGATAAAACG(+AAAAAA)GATCCTCAGAGAGCAGGTCACCACCACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606121925_t58t TACATAGGAGTGGTGGTGACCTGCTCTCTGAG(+AAAAAA)GATCCGTTTTATCTGGAATAATTATCAAACA NGG 10 8 17 8 24 0 100 5 10
    240606121923_kpai TTGTTTGCTGTTTTCCATTTCCTGGCTCCATG(+AAAAAA)GTACCATTGTTTGCCAGGGAGCCCCACATGT NGG 10 8 17 8 24 0 100 5 10
    240606121922_lj6v GGGCCACATGTGGGGCTCCCTGGCAAACAATG(+AAAAAA)GTACCATGGAGCCAGGAAATGGAAAACAGCA NGG 10 8 17 8 24 0 100 5 10
    240606121921_quwr TGGAGTGAACAATACTGAGTGGCATATGCTTG(+AAAAAA)GATCCGTCCTCTCTTCTAAGGGTTGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606121919_a0jg AAGTAGGCTCACAACCCTTAGAAGAGAGGACG(+AAAAAA)GATCCAAGCATATGCCACTCAGTATTGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606121918_cjpp TACCACCCTGGGAAAAGTCACATTCGAGGCTG(+AAAAAA)GATCCTACTGAAGTCAAGGCTTGTTCGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606121917_3281 GCTGTTAAACGAACAAGCCTTGACTTCAGTAG(+AAAAAA)GATCCAGCCTCGAATGTGACTTTTCCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606121915_nqan TTGCGTGCCACCCGCAGCCCCCAACCTTGTAG(+AAAAAA)GATCCGGGCGATCCCCATGAGGCTCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606121913_s080 CTGAGTTCAGGGAGCCTCATGGGGATCGCCCG(+AAAAAA)GATCCTACAAGGTTGGGGGCTGCGGGTGGCA NGG 10 8 17 8 24 0 100 5 10