Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121943_fldy ATTATTTAAAGCGCGCACACGGAGAGGAAGGG(+AAAAAA)GATCCGGCCGGGAAGGGGGTGGAGAAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606121941_62m5 TCCCCGCCCTTTCTCCACCCCCTTCCCGGCCG(+AAAAAA)GATCCCCTTCCTCTCCGTGTGCGCGCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606121939_7an2 AGCATAAAACTGCTAATGGAGTTTGCACCATG(+AAAAAA)GATCCCATTAACTCTGAAAATGAAGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606121937_7as8 TCAGGACCTCACTTCATTTTCAGAGTTAATGG(+AAAAAA)GATCCATGGTGCAAACTCCATTAGCAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606121936_jg92 ATAGTTTTATGAATATAGCTTTTATTTCAAAG(+AAAAAA)GTACCCAGCCAAAGATAATTAAAACTCTATT NGG 10 8 17 8 24 0 100 5 10
    240606121934_vmqr AACAAAATAGAGTTTTAATTATCTTTGGCTGG(+AAAAAA)GTACCTTTGAAATAAAAGCTATATTCATAAA NGG 10 8 17 8 24 0 100 5 10
    240606121933_2wl6 AACCACAGCTCTGCTAGTCAGGACAACGTTAG(+AAAAAA)GTACCACTGACGACCCACAGGCCACTCATGC NGG 10 8 17 8 24 0 100 5 10
    240606121932_tlrl ACTCGGCATGAGTGGCCTGTGGGTCGTCAGTG(+AAAAAA)GTACCTAACGTTGTCCTGACTAGCAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606121930_fudi GTGATCTAAAGACACTAATGCATAGTCCTTAG(+AAAAAA)GATCCCCTAAGAAAAAGACCCCAGTTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606121928_l7bv CCTGTATTTTAACTGGGGTCTTTTTCTTAGGG(+AAAAAA)GATCCTAAGGACTATGCATTAGTGTCTTTAG NGG 10 8 17 8 24 0 100 5 10