Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121958_l9mj GCTGCACTTGAGGATAAATACCGGAGCTGTAG(+AAAAAA)GTACCACTGCACCTTGTGTACATGGAACCTA NGG 10 8 17 8 24 0 100 5 10
    240606121957_a303 ATGGATAGGTTCCATGTACACAAGGTGCAGTG(+AAAAAA)GTACCTACAGCTCCGGTATTTATCCTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606121956_mlky GAACTAGTCATAAAGAGTGAGGTTGGTGTGAG(+AAAAAA)GTACCAGACATGTAAGAAACAAGGGGACACA NGG 10 8 17 8 24 0 100 5 10
    240606121954_wad9 GGTGGTGTGTCCCCTTGTTTCTTACATGTCTG(+AAAAAA)GTACCTCACACCAACCTCACTCTTTATGACT NGG 10 8 17 8 24 0 100 5 10
    240606121952_8ydp GCACTGCAAGAAACACTACATTCTACACCTGG(+AAAAAA)GTACCTGTGTGTGTTCACTGTATTTCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606121951_is8l TTACCACAAAGAAATACAGTGAACACACACAG(+AAAAAA)GTACCCAGGTGTAGAATGTAGTGTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606121949_86z0 TTCAATTCAGAGCTTTGGGTGTATTTTCTGGG(+AAAAAA)GATCCACAGCTTTCTATTCAAGCAAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606121948_5q54 AATTGCCCAACTTGCTTGAATAGAAAGCTGTG(+AAAAAA)GATCCCCAGAAAATACACCCAAAGCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606121946_jx72 GTTTCATTCCTTTGAAGCAGCTAGTCATCTTG(+AAAAAA)GATCCTTAGTCAGAATTTGCTCTTTTTATCT NGG 10 8 17 8 24 0 100 5 10
    240606121945_xwdj CTCTAAGATAAAAAGAGCAAATTCTGACTAAG(+AAAAAA)GATCCAAGATGACTAGCTGCTTCAAAGGAAT NGG 10 8 17 8 24 0 100 5 10