Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121911_zpw9 CGCCCTCTAGTGACCGGGAGGCCAGTAACAGG(+AAAAAA)GATCCCTTTCTCCAAATGACCAAATGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606121910_sdzl ATCGACCCAACATTTGGTCATTTGGAGAAAGG(+AAAAAA)GATCCCTGTTACTGGCCTCCCGGTCACTAGA NGG 10 8 17 8 24 0 100 5 10
    240606121908_9x21 ATTTATACATAAAACTCCCAAGGAACCATGTG(+AAAAAA)GATCCGACAGAATGTTAATAGCACATCTAAG NGG 10 8 17 8 24 0 100 5 10
    240606121907_qvq2 TTATTCTTAGATGTGCTATTAACATTCTGTCG(+AAAAAA)GATCCACATGGTTCCTTGGGAGTTTTATGTA NGG 10 8 17 8 24 0 100 5 10
    240606121906_mynq TTCTGTACTGCTTAAGATTCAGCAGGGCACTG(+AAAAAA)GATCCCTGGGAGCTGGAGTTAGGTGACTATG NGG 10 8 17 8 24 0 100 5 10
    240606121903_tmgb CCACTCATAGTCACCTAACTCCAGCTCCCAGG(+AAAAAA)GATCCAGTGCCCTGCTGAATCTTAAGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240606121902_r4tb GCATCAAGTCTACCTTTTGCATTGGTTTTATG(+AAAAAA)GATCCATGTATATAGGTAGCCATCATAAACT NGG 10 8 17 8 24 0 100 5 10
    240606121900_cvqn CCTTCAGTTTATGATGGCTACCTATATACATG(+AAAAAA)GATCCATAAAACCAATGCAAAAGGTAGACTT NGG 10 8 17 8 24 0 100 5 10
    240606121859_2vsg CACGTGACCCTGAACAACAGTGAGCCTCTCGG(+AAAAAA)GATCCAGCCCACCATCACCTTGTTCGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606121857_rkvj CTCATAGCTCCGAACAAGGTGATGGTGGGCTG(+AAAAAA)GATCCCGAGAGGCTCACTGTTGTTCAGGGTC NGG 10 8 17 8 24 0 100 5 10