Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121810_21fh AAGGTTCTCACTGTCGTCAACAATCTTTCTAG(+AAAAAA)GTACCTTTGGATGGTTCTAGCACATTTATCA NGG 10 8 17 8 24 0 100 5 10
    240606121808_qhoj TTGTCTGATAAATGTGCTAGAACCATCCAAAG(+AAAAAA)GTACCTAGAAAGATTGTTGACGACAGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606121807_fqaz GAACCCCTGAACTTGGAAAACTGAGCCAAGAG(+AAAAAA)GATCCCAAGTTCAAAGCCAGCCTGGGCTACA NGG 10 8 17 8 24 0 100 5 10
    240606121805_qkmw TGCTATGTAGCCCAGGCTGGCTTTGAACTTGG(+AAAAAA)GATCCTCTTGGCTCAGTTTTCCAAGTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606121803_zg38 GGGACTGCCTCTAATGTGCGTTTGATTCCCTG(+AAAAAA)GTACCTGACACTGTACCCAGTACACACAAAT NGG 10 8 17 8 24 0 100 5 10
    240606121801_95xa TGTGCATTTGTGTGTACTGGGTACAGTGTCAG(+AAAAAA)GTACCAGGGAATCAAACGCACATTAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606121800_x071 TATATAGACCAGGTCAGGCCTGAATTCACAGG(+AAAAAA)GATCCACCTGCCTCTGTCTCCCTGCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606121758_31g2 TGATCTCAGCAGCAGGGAGACAGAGGCAGGTG(+AAAAAA)GATCCCTGTGAATTCAGGCCTGACCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606121756_56hs GTGATTTAATCATGATTGTAAAAATTGTGTGG(+AAAAAA)GTACCTTAAAAAAAAACTGCTTCTGTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606121755_xx4v GCGACCAAAGACAGAAGCAGTTTTTTTTTAAG(+AAAAAA)GTACCCACACAATTTTTACAATCATGATTAA NGG 10 8 17 8 24 0 100 5 10