Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121738_62q3 CTAATACAGGTATCTAGAATGTGAACTGAGTG(+AAAAAA)GATCCAAAAGCTTCACACAGAGGAGAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606121736_80d7 TAGATCTTGCTCTCCTCTGTGTGAAGCTTTTG(+AAAAAA)GATCCACTCAGTTCACATTCTAGATACCTGT NGG 10 8 17 8 24 0 100 5 10
    240606121735_82rn GGGGGGAAAGGAAAAAAAACCTAAGGGCTTTG(+AAAAAA)GTACCTGGGCTATCATTCCTCTACCACGTTT NGG 10 8 17 8 24 0 100 5 10
    240606121733_319y ATAAGAAACGTGGTAGAGGAATGATAGCCCAG(+AAAAAA)GTACCAAAGCCCTTAGGTTTTTTTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606121731_2y7r AGTGGCCAGAGGGAAGTGGATACGAGGGTAGG(+AAAAAA)GTACCAGGTTGGTCTGGAATTCTGTCAGATC NGG 10 8 17 8 24 0 100 5 10
    240606121729_j84n ATGTTGATCTGACAGAATTCCAGACCAACCTG(+AAAAAA)GTACCCTACCCTCGTATCCACTTCCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606121728_rvze AGGATTGGAAACACACTGCCTAGACAAAAATG(+AAAAAA)GTACCCTGTGTTCTCTGCGTTCCTCACGTCT NGG 10 8 17 8 24 0 100 5 10
    240606121726_9lcd TAGCCAGACGTGAGGAACGCAGAGAACACAGG(+AAAAAA)GTACCATTTTTGTCTAGGCAGTGTGTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606121725_9gjn AGTACTGGCAGTAGCCAGGGAAATCCCATTTG(+AAAAAA)GATCCAAAGGCCAGCAGTTAGGAAAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606121723_lnza TGACCTTCCTCTTTCCTAACTGCTGGCCTTTG(+AAAAAA)GATCCAAATGGGATTTCCCTGGCTACTGCCA NGG 10 8 17 8 24 0 100 5 10