Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121632_5wpk ATTTTGCTTCATGTATCCAGGTCCTCTGCAGG(+AAAAAA)GTACCAAGGAATGTTCTTCAGGATAGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240606121629_le4s AATTGTTCCGCTATCCTGAAGAACATTCCTTG(+AAAAAA)GTACCCTGCAGAGGACCTGGATACATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606121628_54yj GTCAAAGTATGTTTAACATATGATTTCTTCAG(+AAAAAA)GATCCCTCCCCTTTCTGAATTCCAAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240606121626_bb9s AGTTCCTCCATTTGGAATTCAGAAAGGGGAGG(+AAAAAA)GATCCTGAAGAAATCATATGTTAAACATACT NGG 10 8 17 8 24 0 100 5 10
    240606121624_zt0m TTCTGCCACTCCCTCATGCATGTTTGAGGGTG(+AAAAAA)GATCCCATCCAATGACCAGTAAGAGATTAAA NGG 10 8 17 8 24 0 100 5 10
    240606121623_8jxo AAGCTTTTAATCTCTTACTGGTCATTGGATGG(+AAAAAA)GATCCACCCTCAAACATGCATGAGGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606121621_mj0c CCACCCCAGCCAAGAGCTGAGGCGCATGAGAG(+AAAAAA)GATCCTGAGCATCCCCAGAAGGGTGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606121620_qzhc CACAGCAGACACACCCTTCTGGGGATGCTCAG(+AAAAAA)GATCCTCTCATGCGCCTCAGCTCTTGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606121618_7cd5 CAGGTGGCTAACAACTGCCTTTAACTTCAAGG(+AAAAAA)GATCCAAAGCCCTCCTCAGGCACCTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240606121617_4v47 TATGTGGGTACAGGTGCCTGAGGAGGGCTTTG(+AAAAAA)GATCCCTTGAAGTTAAAGGCAGTTGTTAGCC NGG 10 8 17 8 24 0 100 5 10