Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121537_8ir6 GCAAATATTTGCTCTTTTCCCAGATTGTCCTG(+AAAAAA)GTACCTACCCCTTGTACACATCCCTGCCTAT NGG 10 8 17 8 24 0 100 5 10
    240606121536_qssw CTTTTATAGGCAGGGATGTGTACAAGGGGTAG(+AAAAAA)GTACCAGGACAATCTGGGAAAAGAGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240606121534_hud2 TTCTTCATGAACGATATTTGACATGTGCTTTG(+AAAAAA)GTACCCTTCTCTGAAAGTTGAAAACCTACCT NGG 10 8 17 8 24 0 100 5 10
    240606121533_a1oq TAAGTAGGTAGGTTTTCAACTTTCAGAGAAGG(+AAAAAA)GTACCAAAGCACATGTCAAATATCGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240606121531_osws AGAAAGGGCTGCAGTCAGTTTGACTAAAACGG(+AAAAAA)GATCCACGTTATTGCTTAGAAAGGAAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606121530_drsk GCTGGCTTGCTTCCTTTCTAAGCAATAACGTG(+AAAAAA)GATCCCGTTTTAGTCAAACTGACTGCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606121528_mj6a AGTATAGTATCAATAACGTCTACATCACCAGG(+AAAAAA)GTACCTTTGCATTAAATGAGTTTTGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606121527_1r97 TTACTATTTTGCAAAACTCATTTAATGCAAAG(+AAAAAA)GTACCCTGGTGATGTAGACGTTATTGATACT NGG 10 8 17 8 24 0 100 5 10
    240606121525_qtxo CAAAGATTAAAGAGGACCAAAAGATGAGTTCG(+AAAAAA)GTACCATAACAAAAAAAATCTCACCTACTGT NGG 10 8 17 8 24 0 100 5 10
    240606121524_5qjx ATGGTACAGTAGGTGAGATTTTTTTTGTTATG(+AAAAAA)GTACCGAACTCATCTTTTGGTCCTCTTTAAT NGG 10 8 17 8 24 0 100 5 10