Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121508_rxrt CACAATTTTCCTTTTCATCCCCCACCACATTG(+AAAAAA)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240606121507_00zv CCTCTACGAGAAAAGTGATGGTATATATTTAG(+AAAAAA)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606121505_btj0 ACTGTCTGGGTTTGGTGACTGATGATGGAATG(+AAAAAA)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606121503_l6js GGAATGACCATCCAGAGACTGCTCTACCTGGG(+AAAAAA)GATCCATTCCATCATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606121501_2fo8 TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+AAAAAA)GATCCTCCTGTAGTACTATGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606121500_aab8 TACTCAGAAAATAGTTCATAGTACTACAGGAG(+AAAAAA)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240606121459_0l8i CACAATTTTCCTTTTCATCCCCCACCACATTG(+AAAAAA)GATCCTAAATATATACCATCACTTTTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606121457_g9hc CCTCTATGAGAAAAGTGATGGTATATATTTAG(+AAAAAA)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606121456_naxz GTAAAAGGTCTACAATACCAGATGCGAGTCGG(+AAAAAA)GATCCAAATCACCCAGCCTAACTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606121455_fan0 TGTAGCTAAGCACAGTTAGGCTGGGTGATTTG(+AAAAAA)GATCCCGACTCGCATCTGGTATTGTAGACCT NGG 10 8 17 8 24 0 100 5 10