Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121438_0c85 GTTAGTAAATAAGATGAGGTTAGCACTTTTAG(+AAAAAA)GTACCAGTTGTCCTGAGTAACTGTACTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606121437_0xvw CTCTGTTGGAGTACAGTTACTCAGGACAACTG(+AAAAAA)GTACCTAAAAGTGCTAACCTCATCTTATTTA NGG 10 8 17 8 24 0 100 5 10
    240606121435_p36h AAGTAATCGGGAGCCTTTGATTTGGCAGCCGG(+AAAAAA)GTACCCTGGTGCTTTACAGTAGCACATGGCC NGG 10 8 17 8 24 0 100 5 10
    240606121432_r2s7 GGAGGGGCCATGTGCTACTGTAAAGCACCAGG(+AAAAAA)GTACCCGGCTGCCAAATCAAAGGCTCCCGAT NGG 10 8 17 8 24 0 100 5 10
    240606121430_u78z GCACTGCAGAAAGCATCTGAAAATCGTCCTTG(+AAAAAA)GATCCCATGTGAGTGGTGGCACCACTAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606121429_g2vn GCAGAGACTTAGTGGTGCCACCACTCACATGG(+AAAAAA)GATCCAAGGACGATTTTCAGATGCTTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606121427_1ol6 GTCAAGTTCACCATGTGCTTAAATAACAGATG(+AAAAAA)GATCCTAGGCTAGAGGGTTTTCTCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606121426_xqe2 AGCTTTGCTGAGGAGAAAACCCTCTAGCCTAG(+AAAAAA)GATCCATCTGTTATTTAAGCACATGGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240606121424_gkbn AAACACCTGTTAGTTCTAACTGTGTAGATTGG(+AAAAAA)GTACCATAGTTAAGCTTAAGGATCCGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606121423_kq4c CGTTCACACCCGGATCCTTAAGCTTAACTATG(+AAAAAA)GTACCCAATCTACACAGTTAGAACTAACAGG NGG 10 8 17 8 24 0 100 5 10