Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121523_7b57 ACTGTCTGGGTTTGGTGACTGATAATGGAATG(+AAAAAA)GATCCCCAGTTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606121521_7e7k GGAATGACCATCCAGAGACTGCTCTAACTGGG(+AAAAAA)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606121520_akft TTCTGGGTATGTGTCCAGGAGCATAATTGCTG(+AAAAAA)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606121518_xyvw TACTCAGAAAATAGTTCACAGTACTACAGGAG(+AAAAAA)GATCCAGCAATTATGCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240606121517_6ld9 CACAATTTTCCTTTTCATCCCCCACCACATTG(+AAAAAA)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240606121516_thw4 CCTCTACGAGAAAAGTGATGGTATATATTTAG(+AAAAAA)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606121515_gfj0 ACTGTCTGGGTTTGGTGACTGATAATGGAATG(+AAAAAA)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606121512_ub9n GGAATGACCATCCAGAGACTGCTCTACCTGGG(+AAAAAA)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606121511_p4sg TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+AAAAAA)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606121509_y4wz TACTCAGAAAATAGTTCACAGTACTACAGGAG(+AAAAAA)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10