Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121422_be7f TGTGTAGATTGGGTACCATAGTTAAGCTTAAG(+AAAAAA)GATCCGGGTGTGAACGGCACACAAGCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606121419_62n0 AGAAGCCCCAGCTTGTGTGCCGTTCACACCCG(+AAAAAA)GATCCTTAAGCTTAACTATGGTACCCAATCT NGG 10 8 17 8 24 0 100 5 10
    240606121418_eaoy GCGAGGGGACACAGCAACCGTGCAAACGTCAG(+AAAAAA)GATCCTGCATTCTTTAGATTGTTTCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606121417_k4x9 TAATTTAAAAGGAAACAATCTAAAGAATGCAG(+AAAAAA)GATCCTGACGTTTGCACGGTTGCTGTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606121415_lzit TAAAGTATTTGCCATGCAAGCAGGAAGACCTG(+AAAAAA)GATCCTCATAGAAAGAAGGCAGGCAACATCA NGG 10 8 17 8 24 0 100 5 10
    240606121413_13ra CCATCTGATGTTGCCTGCCTTCTTTCTATGAG(+AAAAAA)GATCCAGGTCTTCCTGCTTGCATGGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240606121412_0ct0 ATGAGAAAGAAACACGGCAACACACTCTATTG(+AAAAAA)GTACCTGTGTAAGTTAAAGTTCAGGGTCGAC NGG 10 8 17 8 24 0 100 5 10
    240606121411_c2lf GTTCAGTCGACCCTGAACTTTAACTTACACAG(+AAAAAA)GTACCAATAGAGTGTGTTGCCGTGTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606121409_v3mm GATGCACCAGGTGAACCTTGGGCATCTAACTG(+AAAAAA)GATCCTCATTTATTACCAAAGATAGTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606121408_msxa TATGAAACCAACTATCTTTGGTAATAAATGAG(+AAAAAA)GATCCAGTTAGATGCCCAAGGTTCACCTGGT NGG 10 8 17 8 24 0 100 5 10