Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121319_2yht CAGGTCTTCCCTAGAGTCCTAAGGGCATCGAG(+AAAAAA)GATCCCATCTGCCATGTTTCAGTCTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606121318_86jn CAGTGGAGGGCAGACTGAAACATGGCAGATGG(+AAAAAA)GATCCTCGATGCCCTTAGGACTCTAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606121316_qm3l TGTATGATCTGGGACAGGCTGAAGCAGGCAGG(+AAAAAA)GTACCCACTCTGCAGTGTAGTTCCTGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240606121314_b4i9 CTTCTCGCTCCAGGAACTACACTGCAGAGTGG(+AAAAAA)GTACCCTGCCTGCTTCAGCCTGTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240606121313_e5g5 GTTACACCTAGGCTGCAGGAAGGATGCTGGAG(+AAAAAA)GTACCCTGGCCTCACCTGGTTTAGGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240606121311_01uj CACATTCCTGTCCTAAACCAGGTGAGGCCAGG(+AAAAAA)GTACCTCCAGCATCCTTCCTGCAGCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606121310_lgvv ACGGCCCCAAGAAATGCTGAGATTTTACAATG(+AAAAAA)GATCCATACTCCATTAAGAGATGTGTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606121308_4eme GTATGAACTCACACATCTCTTAATGGAGTATG(+AAAAAA)GATCCATTGTAAAATCTCAGCATTTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606121307_e2s2 ACCCCCTGCAGTGGGCTAATTAGGACTGAGGG(+AAAAAA)GTACCTCTAAGGCTTTGGGGGTTCAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240606121305_r1xu GAGGCCTCTGTTGAACCCCCAAAGCCTTAGAG(+AAAAAA)GTACCCCTCAGTCCTAATTAGCCCACTGCAG NGG 10 8 17 8 24 0 100 5 10