Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121248_l3cc AGGGGAGCTTGTGATTGCTATGCCTTCAACAG(+AAAAAA)GATCCCTCAGGGTCTCATCTTGAAGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606121247_5jmd AGCCCTCCACTCTTCAAGATGAGACCCTGAGG(+AAAAAA)GATCCTGTTGAAGGCATAGCAATCACAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606121245_tf0k GCTTACATTTACTGGCCCTGAAGCTTCCAAAG(+AAAAAA)GATCCGCATAAAAACCTAGAGAAAGAATAAG NGG 10 8 17 8 24 0 100 5 10
    240606121243_6p6r CTTCCCTTATTCTTTCTCTAGGTTTTTATGCG(+AAAAAA)GATCCTTTGGAAGCTTCAGGGCCAGTAAATG NGG 10 8 17 8 24 0 100 5 10
    240606121242_2xw7 TCCTAGGAGGCCTTTGTTTTCCCAAATTCAAG(+AAAAAA)GATCCAAAAATTCTTCAGGTCAGAAAACTAA NGG 10 8 17 8 24 0 100 5 10
    240606121241_gune AAAGTTTAGTTTTCTGACCTGAAGAATTTTTG(+AAAAAA)GATCCTTGAATTTGGGAAAACAAAGGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606121240_u5hp GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+AAAAAA)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606121238_941v AATAGTTTTATTTCACACCTTGTTTCTAGCTG(+AAAAAA)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240606121237_zsku ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+AAAAAA)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240606121235_38gk TTACACACTACCAAGTCCAGCCACAGCACAAG(+AAAAAA)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10