Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121352_d5uq GTCCCAGTTTCCACCTTCCTCTGGCCCAGTAG(+AAAAAA)GATCCCCAATCTGACTCTTTCAAGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606121350_yews GAATGTCTGAGCCTTGAAAGAGTCAGATTGGG(+AAAAAA)GATCCTACTGGGCCAGAGGAAGGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240606121348_dkbp GGTAACCACATTTTTCAACTCCACCCACCATG(+AAAAAA)GTACCCTAGAACCAAGACTGGAATACTGACT NGG 10 8 17 8 24 0 100 5 10
    240606121347_z8ev TCTCCAGTCAGTATTCCAGTCTTGGTTCTAGG(+AAAAAA)GTACCATGGTGGGTGGAGTTGAAAAATGTGG NGG 10 8 17 8 24 0 100 5 10
    240606121345_ilgy ACCACATGGTGGCCCACAACCATCTGTAATGG(+AAAAAA)GATCCAATGCCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606121344_mj7u GCTGTCTTCAGACACACCAAAAGAGGGCATTG(+AAAAAA)GATCCCATTACAGATGGTTGTGGGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606121343_1li0 TTAGAGTGGTGGGTTGGTCAGCTGGAAGACAG(+AAAAAA)GATCCAAAATGTTCCATGGAAGGCTGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606121341_bqmz AGCCTAAAGACAGCCTTCCATGGAACATTTTG(+AAAAAA)GATCCTGTCTTCCAGCTGACCAACCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240606121340_s9s2 GCTGAGGGGCTGAGTTCCCTTGAAGCAGCCAG(+AAAAAA)GTACCGGTGGCCAGCTCTCTCTTGTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606121337_gh9r TGGACTCTAGAACAAGAGAGAGCTGGCCACCG(+AAAAAA)GTACCTGGCTGCTTCAAGGGAACTCAGCCCC NGG 10 8 17 8 24 0 100 5 10