Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121646_fnly GGATTGAACTCAAATTGCTAGGTCGGCAGCAG(+AAAAAA)GTACCTTTACCCACTGGACCATCTAGTTGTC NGG 10 8 17 8 24 0 100 5 10
    240606121645_082w AAGGTGACAACTAGATGGTCCAGTGGGTAAAG(+AAAAAA)GTACCTGCTGCCGACCTAGCAATTTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606121643_mofn ACAAGAAGATACCTGAGAGAAGCCATGGGGAG(+AAAAAA)GATCCAGCATTGATGGTGTAGCAGAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606121642_df1k GGCCTCTGGCTTCTGCTACACCATCAATGCTG(+AAAAAA)GATCCTCCCCATGGCTTCTCTCAGGTATCTT NGG 10 8 17 8 24 0 100 5 10
    240606121640_m0p9 GTTCTCAAAACAAATGTTCTTAGTTCTCAGTG(+AAAAAA)GATCCTGCAGATCTTAAATCATTCTTTGCGT NGG 10 8 17 8 24 0 100 5 10
    240606121639_f87i TAAAAACGCAAAGAATGATTTAAGATCTGCAG(+AAAAAA)GATCCACTGAGAACTAAGAACATTTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606121638_283z GCAGTCTTTTTAGCAGTAGACTCAGAAGTCAG(+AAAAAA)GATCCTCAGACCAACTCAATATATACTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606121637_gvmd AAAACTAAAAGTATATATTGAGTTGGTCTGAG(+AAAAAA)GATCCTGACTTCTGAGTCTACTGCTAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606121635_ebsv TTGGCTGGTACTTCCTTCCTTCCAGGAGATGG(+AAAAAA)GATCCCTCTAGATACAGGGCCTAGAGACCAA NGG 10 8 17 8 24 0 100 5 10
    240606121633_lryt AGGTTTTGGTCTCTAGGCCCTGTATCTAGAGG(+AAAAAA)GATCCCATCTCCTGGAAGGAAGGAAGTACCA NGG 10 8 17 8 24 0 100 5 10