Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121840_4xhc GCTGTTTCAAGAGCATTAGGTACATGCACATG(+AAAAAA)GTACCTTCCTAATTAATTGACCTGGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606121839_os4v CAGCTCTTTTTCCAGGTCAATTAATTAGGAAG(+AAAAAA)GTACCATGTGCATGTACCTAATGCTCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606121837_h3ju ATAACTGTTAACTGCAACATATCTTTGGCAGG(+AAAAAA)GATCCTCAGTCGTACATTTTGTCTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240606121836_x8y4 AGAGGCATTTAAAGACAAAATGTACGACTGAG(+AAAAAA)GATCCCTGCCAAAGATATGTTGCAGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240606121834_7kls TCTGAGAGCAGTGTAAACCTCACAGGGCTTTG(+AAAAAA)GATCCCCTCGGACCCCCTTGGACACAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606121832_ih2g CCTTCTCCTCTGTGTCCAAGGGGGTCCGAGGG(+AAAAAA)GATCCAAAGCCCTGTGAGGTTTACACTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606121831_lqgc CAGTCCTACTGGAGATTCATTTTTGGCATCAG(+AAAAAA)GTACCTGAGTTATTAACTTCATTCATTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606121830_23wx CAGCTATTTAATGAATGAAGTTAATAACTCAG(+AAAAAA)GTACCTGATGCCAAAAATGAATCTCCAGTAG NGG 10 8 17 8 24 0 100 5 10
    240606121828_anxt TTCGTGCACACAGTGGCCAGAAAAGGGTATTG(+AAAAAA)GATCCCTTGGAGCTAGACTTACAAAGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606121826_rjwe GCTTACACCCCTTTGTAAGTCTAGCTCCAAGG(+AAAAAA)GATCCAATACCCTTTTCTGGCCACTGTGTGC NGG 10 8 17 8 24 0 100 5 10