Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122059_381y AAGAAACAGTCTTTTTATAGCAAGCCAGACTG(+AAAAAA)GTACCTGGCTGTTCCTAGGTAACTGTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606122057_w7n8 GCTCCACCCAACAGTTACCTAGGAACAGCCAG(+AAAAAA)GTACCAGTCTGGCTTGCTATAAAAAGACTGT NGG 10 8 17 8 24 0 100 5 10
    240606122056_p1be TAGATCGTTAACCTGATCCTGATTTAACTTTG(+AAAAAA)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606122054_iqj7 ACGAAATGGACAAATTTCTGGACAGATACCAG(+AAAAAA)GTACCAAAGTTAAATCAGGATCAGGTTAACG NGG 10 8 17 8 24 0 100 5 10
    240606122053_ftbu CAAATGGTTTATTTTAAACTAGACCCATCATG(+AAAAAA)GTACCTCAGGGAAAGATGTCTCAGCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606122051_nsp0 AGTGGGTCCATGCTGAGACATCTTTCCCTGAG(+AAAAAA)GTACCATGATGGGTCTAGTTTAAAATAAACC NGG 10 8 17 8 24 0 100 5 10
    240606122050_orzl TACCCAGGGAGATGCGAGGAGATAGATTCATG(+AAAAAA)GTACCTGAGCACAGGTAACCAGCCACATGGT NGG 10 8 17 8 24 0 100 5 10
    240606122049_24jp ATTCTACCATGTGGCTGGTTACCTGTGCTCAG(+AAAAAA)GTACCATGAATCTATCTCCTCGCATCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606122047_f5mw GGCTAACTGTAGCTGTATGTTAACAGTGGCAG(+AAAAAA)GTACCCAGCTCCTCTCCCTGAGGCTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240606122046_r1l6 ATGGCAGCTGTAGCCTCAGGGAGAGGAGCTGG(+AAAAAA)GTACCTGCCACTGTTAACATACAGCTACAGT NGG 10 8 17 8 24 0 100 5 10