Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174210_brsb CATAAATCTGGGAAGAAATGGCGGTGCAGTGG(+AGGACA)GATCCCAGAAACGAAGCCTCCACCTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613174209_7tph GAAAAACAATGACTGGAATATCATTGGAGATG(+AGGACA)GATCCCAGGTAGGGGTTACAATTAGCCATGG NGG 10 8 17 8 24 0 100 5 10
    240613174206_3pkq ACAAACCATGGCTAATTGTAACCCCTACCTGG(+AGGACA)GATCCATCTCCAATGATATTCCAGTCATTGT NGG 10 8 17 8 24 0 100 5 10
    240613174205_6b0y CACAGCACAAACTACCAAATTTTGATTTTTTG(+AGGACA)GATCCCAGCAATAATTTTTAATAGTTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240613174203_ip51 CCAGTTTGAAAACTATTAAAAATTATTGCTGG(+AGGACA)GATCCAAAAAATCAAAATTTGGTAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613174202_h0h3 ACCCATCTCAGGTTATAGTCCACATAAGCGTG(+AGGACA)GATCCTACATGTTTCTTGATTAGGATAGTCT NGG 10 8 17 8 24 0 100 5 10
    240613174201_ddes TAGGTAGACTATCCTAATCAAGAAACATGTAG(+AGGACA)GATCCACGCTTATGTGGACTATAACCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613174159_un4u TGCCTGTACCTTCAAATGTAAAGTAAGCCACG(+AGGACA)GTACCTTGTGAGACATGTGTTCCTTGTGTTC NGG 10 8 17 8 24 0 100 5 10
    240613174158_osst CCAAGGAACACAAGGAACACATGTCTCACAAG(+AGGACA)GTACCGTGGCTTACTTTACATTTGAAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613174156_p55r AGGTGACGGTCTGCTCATTTGTCAATGGGATG(+AGGACA)GTACCTTCCTTTCTCTTCATGGATTGCATCC NGG 10 8 17 8 24 0 100 5 10